I am doing topo directional cloning using pENTR Directional TOPO Cloning Kits. I am using manufacturers protocol to do transformation. the colony coming normally on kanamycin plates. but while screeing the colonies I am unable to get positive colonies which are amplyfying. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
user guide For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. pENTR Directional TOPO Cloning Kits Fiveminute, directional TOPO Cloning of bluntend PCR products into an entry vector We offer two types of TOPO vectors that offer easy TOPO cloning to create a Gateway Entry vector.
pCR8GWTOPO vector kits and pENTRDTOPO vector families both offer 5minute, efficient TOPO cloning, with 95 efficiency. pentrdtopo DNASU Gateway type (lambda atttype) recombinational cloning system entry (master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning.
May 20, 2015 Chapolin Dr. Chapatn y Topo Gigio (El besito de las buenas noches) Indito (1979) TOPO GIGIO Es mi mam" Quiero ser como mi pap" A la camita Gateway(TM) Directional TOPO(TM) entry vector. Features attL recombination sites for efficient recombination with a Gateway(TM) destination vector, universal M13 sites to facilitate sequencing, pUCbased ori for high plasmid yields, a gene 10 sequence and a ShineDelgano sequence for efficient initiation of native expression or Cterminal The pENTRDTOPO Cloning Kits utilize a highly efficient, 5minute cloning strategy (TOPO Cloning) to directionally clone a bluntend PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System.
Bluntend PCR products clone directionally at greater than 90 efficiency Is anyone familiar with PENTR D TOPO cloning? I have been trying to generate clones using pENTR DTOPO for gateway cloning. I am able to amplify my insert, set up a reaction and get colonies.