SYBR Green qPCR 2X Master Mix is ideal for highthroughput realtime PCR screening and validation. Provides affordable qPCR and robust results! The FastStart Universal SYBR Green Master (ROX) is a readytouse, 2x concentrated master mix that contains all the reagents (except primers and template) needed for running realtime DNA detection assays, including qPCR and Visit the web site to verify that you are using the most current version of this Technical Manual.
(dsDNA) than SYBR Green I. GoTaq qPCR Master Mix is provided as a simpletouse, Select SYBR or FAM as the SYBR Select Master Mix User Guide Observation Rn vs. Cycle plot is not displayed Extremely high Rn or Rn values Lower Rn values obtained in early cycles High variability across the reaction plate Possible Cause ROX dye was not selected as the passive reference when the plate document was set up ROX dye was not selected as the passive SYBR Select Master Mix for CFX User Guide 11 Methods Procedural Overview This diagram is an overview of the procedures for performing gene expression experiments.
12 SYBR Select Master Mix for CFX User Guide Prepare the Template Examine RNA Template Quality After isolating the template, examine its quality and quantity and store it properly.
2 SYBR Select Master Mix User Guide For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. vii Safety Fast SYBR Green Master Mix Protocol b. Select the language of your choice. c. Click Search. 3. To view, download, or print the document of interest: a. Rightclick the document title. SYBR Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems realtime PCR instruments except the 7900HT realtime PCR system.
It is also compatible with the BioRad IQ5, Roche LightCycler LC480, and Stratagene MX3005P systems. The Power SYBR Green PCR Master Mix and RTPCR Protocol See your instrument users manual for detailed instructions on how to configure the plate documents.
Click on Navigator button, select the template folder, click Import and select the file